Immunohistochemistry:Article Title: CCL11 promotes hepatocellular carcinoma recurrence after surgery by potentiating immunosuppressive CCR5 + CD206 + M2-like macrophages and promoting tumor invasiveness
Article Snippet: .. Antibodies used for immunohistochemistry (IHC) and immunofluorescence (IF) included: anti-human CCL11 (#MAB320-SP) and anti-human/mouse CCR5 (#MAB182-SP) from R&D Systems; anti-human/mouse CD206 (#24595) from Cell Signaling Technology; and anti-human Mafk (#PA5-115534) and anti-human MMP13 (#MA5-43722) from Invitrogen. .. Antibodies used for flow cytometry analysis were purchased from Biolegend including anti-human CCR5 (AF700, #359115), anti-human CD68 (BV421, #333827), anti-human CD206 (PE, #321105), anti-human CD86 (APC, #374207), anti-mouse CD45 (BV510, #103137), anti-mouse CD11b (APC-CY7, #101225), anti-mouse F4/80 (AF700, #123130), anti-mouse CD206 (FITC, #141703), anti-mouse CCR5 (APC, #107011), anti-mouse PDL1 (BV650, #124336), anti-mouse CD3 (APC-CY7, #100221), anti-mouse CD4 (FITC, #116003), anti-mouse CD25 (AF700, #102024), anti-mouse Foxp3 (AF647, #126407), and anti-mouse CD8 (APC, #100711).
Article Title: CCL11 promotes hepatocellular carcinoma recurrence after surgery by potentiating immunosuppressive CCR5 + CD206 + M2-like macrophages and promoting tumor invasiveness.
Article Snippet: .. Antibodies and reagents Antibodies used for immunohistochemistry (IHC) and immunofluorescence (IF) included: anti-human CCL11 (#MAB320-SP) and anti-human/mouse CCR5 (#MAB182-SP) from R&D Systems; anti-human/mouse CD206 (#24595) from Cell Signaling Technology; and anti-human Mafk (#PA5-115534) and anti-human MMP13 (#MA5-43722) from Invitrogen. .. Antibodies used for flow cytometry analysis were purchased from Biolegend including anti-human CCR5 (AF700, #359115), anti-human CD68 (BV421, #333827), anti-human CD206 (PE, #321105), anti-human CD86 (APC, #374207), anti-mouse CD45 (BV510, #103137), anti-mouse CD11b (APC-CY7, #101225), anti-mouse F4/80 (AF700, #123130), anti-mouse CD206 (FITC, #141703), anti-mouse CCR5 (APC, #107011), anti-mouse PDL1 (BV650, #124336), anti-mouse CD3 (APC-CY7, #100221), anti-mouse CD4 (FITC, #116003), anti-mouse CD25 (AF700, #102024), anti-mouse Foxp3 (AF647, #126407), and anti-mouse CD8 (APC, #100711).
Immunofluorescence:Article Title: CCL11 promotes hepatocellular carcinoma recurrence after surgery by potentiating immunosuppressive CCR5 + CD206 + M2-like macrophages and promoting tumor invasiveness
Article Snippet: .. Antibodies used for immunohistochemistry (IHC) and immunofluorescence (IF) included: anti-human CCL11 (#MAB320-SP) and anti-human/mouse CCR5 (#MAB182-SP) from R&D Systems; anti-human/mouse CD206 (#24595) from Cell Signaling Technology; and anti-human Mafk (#PA5-115534) and anti-human MMP13 (#MA5-43722) from Invitrogen. .. Antibodies used for flow cytometry analysis were purchased from Biolegend including anti-human CCR5 (AF700, #359115), anti-human CD68 (BV421, #333827), anti-human CD206 (PE, #321105), anti-human CD86 (APC, #374207), anti-mouse CD45 (BV510, #103137), anti-mouse CD11b (APC-CY7, #101225), anti-mouse F4/80 (AF700, #123130), anti-mouse CD206 (FITC, #141703), anti-mouse CCR5 (APC, #107011), anti-mouse PDL1 (BV650, #124336), anti-mouse CD3 (APC-CY7, #100221), anti-mouse CD4 (FITC, #116003), anti-mouse CD25 (AF700, #102024), anti-mouse Foxp3 (AF647, #126407), and anti-mouse CD8 (APC, #100711).
Article Title: CCL11 promotes hepatocellular carcinoma recurrence after surgery by potentiating immunosuppressive CCR5 + CD206 + M2-like macrophages and promoting tumor invasiveness.
Article Snippet: .. Antibodies and reagents Antibodies used for immunohistochemistry (IHC) and immunofluorescence (IF) included: anti-human CCL11 (#MAB320-SP) and anti-human/mouse CCR5 (#MAB182-SP) from R&D Systems; anti-human/mouse CD206 (#24595) from Cell Signaling Technology; and anti-human Mafk (#PA5-115534) and anti-human MMP13 (#MA5-43722) from Invitrogen. .. Antibodies used for flow cytometry analysis were purchased from Biolegend including anti-human CCR5 (AF700, #359115), anti-human CD68 (BV421, #333827), anti-human CD206 (PE, #321105), anti-human CD86 (APC, #374207), anti-mouse CD45 (BV510, #103137), anti-mouse CD11b (APC-CY7, #101225), anti-mouse F4/80 (AF700, #123130), anti-mouse CD206 (FITC, #141703), anti-mouse CCR5 (APC, #107011), anti-mouse PDL1 (BV650, #124336), anti-mouse CD3 (APC-CY7, #100221), anti-mouse CD4 (FITC, #116003), anti-mouse CD25 (AF700, #102024), anti-mouse Foxp3 (AF647, #126407), and anti-mouse CD8 (APC, #100711).
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